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blunt end cloning vector pjet1 2  (Addgene inc)


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    Addgene inc blunt end cloning vector pjet1 2
    Blunt End Cloning Vector Pjet1 2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+1+vector/NHR1-Flag+pcDNA3%2E1+(Plasmid+%2317315)/pmc12804166-56-8-12
    Average 94 stars, based on 2 article reviews
    blunt end cloning vector pjet1 2 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Construct:

    Article Title: Protein Kinase A and assembly of an ABCC4 protein network.
    Article Snippet: Avi-ABCC4 construct was generated by cloning the human ABCC4 cDNA into pReceiver - M48 vector (GeneCopoeia) containing the insert N-Avi and IRES biotin ligase. .. APEX-ABCC4 construct was generated by cloning human ABCC4 gene into pCDNA3.1 vector containing the insert flag and APEX (Addgene). .. AR TI CL E IN P RE SS ARTICLE IN PRESS PDZ variant for ABCC4 in these constructs was developed by introducing single mutation that leads to an early stop codon before the PDZ motif using a QuikChange II XL site-directed mutagenesis kit (Stratagene).

    Article Title: Visualization of lysosomal membrane proteins by cryo electron tomography.
    Article Snippet: The sequences for human TRPML1 and human TRPML1-mNeonGreen1D4 were cloned into the mammalian expression vector pcDNA3.1 by Genscript. .. The pcDNA3.1 vector containing human TMEM192mCherry-1D4 was constructed by standard molecular cloning. pLJC5Tmem192-3xHA was a gift from David Sabatini (Addgene plasmid # 102930; (http://n2t.net/addgene:102930); RRID:Addgene_102930)21. ..

    Article Title: Visualization of lysosomal membrane proteins by cryo electron tomography
    Article Snippet: The sequences for human TRPML1 and human TRPML1-mNeonGreen-1D4 were cloned into the mammalian expression vector pcDNA3.1 by Genscript. .. The pcDNA3.1 vector containing human TMEM192-mCherry-1D4 was constructed by standard molecular cloning. pLJC5-Tmem192-3xHA was a gift from David Sabatini (Addgene plasmid # 102930; ( http://n2t.net/addgene:102930 ); RRID:Addgene_102930) . ..

    Article Title: Protein Kinase A and assembly of an ABCC4 protein network
    Article Snippet: Avi-ABCC4 construct was generated by cloning the human ABCC4 cDNA into pReceiver-M48 vector (GeneCopoeia) containing the insert N-Avi and IRES biotin ligase. .. APEX-ABCC4 construct was generated by cloning the human ABCC4 gene into pCDNA3.1 vector containing the insert flag and APEX (Addgene). .. ΔPDZ variant for ABCC4 in these constructs was developed by introducing a single mutation that leads to an early stop codon before the PDZ motif using a QuikChange II XL site-directed mutagenesis kit (Stratagene).

    Generated:

    Article Title: Protein Kinase A and assembly of an ABCC4 protein network.
    Article Snippet: Avi-ABCC4 construct was generated by cloning the human ABCC4 cDNA into pReceiver - M48 vector (GeneCopoeia) containing the insert N-Avi and IRES biotin ligase. .. APEX-ABCC4 construct was generated by cloning human ABCC4 gene into pCDNA3.1 vector containing the insert flag and APEX (Addgene). .. AR TI CL E IN P RE SS ARTICLE IN PRESS PDZ variant for ABCC4 in these constructs was developed by introducing single mutation that leads to an early stop codon before the PDZ motif using a QuikChange II XL site-directed mutagenesis kit (Stratagene).

    Article Title: Protein Kinase A and assembly of an ABCC4 protein network
    Article Snippet: Avi-ABCC4 construct was generated by cloning the human ABCC4 cDNA into pReceiver-M48 vector (GeneCopoeia) containing the insert N-Avi and IRES biotin ligase. .. APEX-ABCC4 construct was generated by cloning the human ABCC4 gene into pCDNA3.1 vector containing the insert flag and APEX (Addgene). .. ΔPDZ variant for ABCC4 in these constructs was developed by introducing a single mutation that leads to an early stop codon before the PDZ motif using a QuikChange II XL site-directed mutagenesis kit (Stratagene).

    Cloning:

    Article Title: Protein Kinase A and assembly of an ABCC4 protein network.
    Article Snippet: Avi-ABCC4 construct was generated by cloning the human ABCC4 cDNA into pReceiver - M48 vector (GeneCopoeia) containing the insert N-Avi and IRES biotin ligase. .. APEX-ABCC4 construct was generated by cloning human ABCC4 gene into pCDNA3.1 vector containing the insert flag and APEX (Addgene). .. AR TI CL E IN P RE SS ARTICLE IN PRESS PDZ variant for ABCC4 in these constructs was developed by introducing single mutation that leads to an early stop codon before the PDZ motif using a QuikChange II XL site-directed mutagenesis kit (Stratagene).

    Article Title: Protein Kinase A and assembly of an ABCC4 protein network
    Article Snippet: Avi-ABCC4 construct was generated by cloning the human ABCC4 cDNA into pReceiver-M48 vector (GeneCopoeia) containing the insert N-Avi and IRES biotin ligase. .. APEX-ABCC4 construct was generated by cloning the human ABCC4 gene into pCDNA3.1 vector containing the insert flag and APEX (Addgene). .. ΔPDZ variant for ABCC4 in these constructs was developed by introducing a single mutation that leads to an early stop codon before the PDZ motif using a QuikChange II XL site-directed mutagenesis kit (Stratagene).

    Plasmid Preparation:

    Article Title: Protein Kinase A and assembly of an ABCC4 protein network.
    Article Snippet: Avi-ABCC4 construct was generated by cloning the human ABCC4 cDNA into pReceiver - M48 vector (GeneCopoeia) containing the insert N-Avi and IRES biotin ligase. .. APEX-ABCC4 construct was generated by cloning human ABCC4 gene into pCDNA3.1 vector containing the insert flag and APEX (Addgene). .. AR TI CL E IN P RE SS ARTICLE IN PRESS PDZ variant for ABCC4 in these constructs was developed by introducing single mutation that leads to an early stop codon before the PDZ motif using a QuikChange II XL site-directed mutagenesis kit (Stratagene).

    Article Title: Mapping the miRNA Landscape of Gallbladder Cancer: Genome-Wide Insights and Functional Implications
    Article Snippet: The collected tissue samples were stored in RNAlater (Invitrogen #AM7020) at –80 till further processing. .. The pcDNA3.1 vector (used as an empty control) was purchased from Addgene (#128034) and miR-17∼92 cloned in pcDNA3.1 vector was kindly provided by Dr. Joshua T. Mendell ( ). ..

    Article Title: DAZAP2 functions as a pan-coronavirus restriction factor by inhibiting viral entry and genomic replication
    Article Snippet: .. Shortly, pcDNA3.1 vector expressing the spike gene of SARS-CoV-2 lacking the C-terminal 21 amino acids, the full spike of SARS-CoV-1, or VSV-G (pMD2.G [Addgene #12259]), was co-transfected in HEK 293T cells with the murine leukemia retrovirus (MLV) expressing the NanoLuc luciferase gene and plasmid expressing the MLV Gag-Pol using Fugene HD transfection reagent (Promega). ..

    Article Title: Visualization of lysosomal membrane proteins by cryo electron tomography.
    Article Snippet: The sequences for human TRPML1 and human TRPML1-mNeonGreen1D4 were cloned into the mammalian expression vector pcDNA3.1 by Genscript. .. The pcDNA3.1 vector containing human TMEM192mCherry-1D4 was constructed by standard molecular cloning. pLJC5Tmem192-3xHA was a gift from David Sabatini (Addgene plasmid # 102930; (http://n2t.net/addgene:102930); RRID:Addgene_102930)21. ..

    Article Title: Visualization of lysosomal membrane proteins by cryo electron tomography
    Article Snippet: The sequences for human TRPML1 and human TRPML1-mNeonGreen-1D4 were cloned into the mammalian expression vector pcDNA3.1 by Genscript. .. The pcDNA3.1 vector containing human TMEM192-mCherry-1D4 was constructed by standard molecular cloning. pLJC5-Tmem192-3xHA was a gift from David Sabatini (Addgene plasmid # 102930; ( http://n2t.net/addgene:102930 ); RRID:Addgene_102930) . ..

    Article Title: METTL3 Methylation Induces Decay of Endogenous Retroelement Transcripts to Promote Tumor Immune Evasion
    Article Snippet: METTL3 (K513R) and SETD1A (R1495A) were generated 176 by a site-directed mutagenesis kit (C214-01, Vazyme). .. The genes of SMYD1-5, 177 SETD7, SETD8 were inserted into pcDNA3.1(+) vector (RRID:Addgene_52535). .. The 178 SETD1A and E2F4 promoter region, spanning nucleotides −2000 to +100 relative to 179 the transcription start site (TSS), was PCR-amplified and ligated into the pGL3-Basic 180 luciferase reporter plasmid (RRID:Addgene_212936).

    Article Title: DAZAP2 functions as a pan-coronavirus restriction factor by inhibiting viral entry and genomic replication.
    Article Snippet: .. Shortly, pcDNA3.1 vector expressing the spike gene of SARS-CoV-2 lacking the C-terminal 21 amino acids, the full spike of SARS-CoV-1, or VSV-G (pMD2.G [Addgene #12259]), was co-transfected in HEK 293T cells with the murine leukemia retrovirus (MLV) expressing the NanoLuc luciferase gene and plasmid expressing the MLV Gag-Pol using Fugene HD transfection reagent (Promega). ..

    Article Title: Protein Kinase A and assembly of an ABCC4 protein network
    Article Snippet: Avi-ABCC4 construct was generated by cloning the human ABCC4 cDNA into pReceiver-M48 vector (GeneCopoeia) containing the insert N-Avi and IRES biotin ligase. .. APEX-ABCC4 construct was generated by cloning the human ABCC4 gene into pCDNA3.1 vector containing the insert flag and APEX (Addgene). .. ΔPDZ variant for ABCC4 in these constructs was developed by introducing a single mutation that leads to an early stop codon before the PDZ motif using a QuikChange II XL site-directed mutagenesis kit (Stratagene).

    Control:

    Article Title: Mapping the miRNA Landscape of Gallbladder Cancer: Genome-Wide Insights and Functional Implications
    Article Snippet: The collected tissue samples were stored in RNAlater (Invitrogen #AM7020) at –80 till further processing. .. The pcDNA3.1 vector (used as an empty control) was purchased from Addgene (#128034) and miR-17∼92 cloned in pcDNA3.1 vector was kindly provided by Dr. Joshua T. Mendell ( ). ..

    Clone Assay:

    Article Title: Mapping the miRNA Landscape of Gallbladder Cancer: Genome-Wide Insights and Functional Implications
    Article Snippet: The collected tissue samples were stored in RNAlater (Invitrogen #AM7020) at –80 till further processing. .. The pcDNA3.1 vector (used as an empty control) was purchased from Addgene (#128034) and miR-17∼92 cloned in pcDNA3.1 vector was kindly provided by Dr. Joshua T. Mendell ( ). ..

    Expressing:

    Article Title: DAZAP2 functions as a pan-coronavirus restriction factor by inhibiting viral entry and genomic replication
    Article Snippet: .. Shortly, pcDNA3.1 vector expressing the spike gene of SARS-CoV-2 lacking the C-terminal 21 amino acids, the full spike of SARS-CoV-1, or VSV-G (pMD2.G [Addgene #12259]), was co-transfected in HEK 293T cells with the murine leukemia retrovirus (MLV) expressing the NanoLuc luciferase gene and plasmid expressing the MLV Gag-Pol using Fugene HD transfection reagent (Promega). ..

    Article Title: DAZAP2 functions as a pan-coronavirus restriction factor by inhibiting viral entry and genomic replication.
    Article Snippet: .. Shortly, pcDNA3.1 vector expressing the spike gene of SARS-CoV-2 lacking the C-terminal 21 amino acids, the full spike of SARS-CoV-1, or VSV-G (pMD2.G [Addgene #12259]), was co-transfected in HEK 293T cells with the murine leukemia retrovirus (MLV) expressing the NanoLuc luciferase gene and plasmid expressing the MLV Gag-Pol using Fugene HD transfection reagent (Promega). ..

    Luciferase:

    Article Title: DAZAP2 functions as a pan-coronavirus restriction factor by inhibiting viral entry and genomic replication
    Article Snippet: .. Shortly, pcDNA3.1 vector expressing the spike gene of SARS-CoV-2 lacking the C-terminal 21 amino acids, the full spike of SARS-CoV-1, or VSV-G (pMD2.G [Addgene #12259]), was co-transfected in HEK 293T cells with the murine leukemia retrovirus (MLV) expressing the NanoLuc luciferase gene and plasmid expressing the MLV Gag-Pol using Fugene HD transfection reagent (Promega). ..

    Article Title: DAZAP2 functions as a pan-coronavirus restriction factor by inhibiting viral entry and genomic replication.
    Article Snippet: .. Shortly, pcDNA3.1 vector expressing the spike gene of SARS-CoV-2 lacking the C-terminal 21 amino acids, the full spike of SARS-CoV-1, or VSV-G (pMD2.G [Addgene #12259]), was co-transfected in HEK 293T cells with the murine leukemia retrovirus (MLV) expressing the NanoLuc luciferase gene and plasmid expressing the MLV Gag-Pol using Fugene HD transfection reagent (Promega). ..

    Transfection:

    Article Title: DAZAP2 functions as a pan-coronavirus restriction factor by inhibiting viral entry and genomic replication
    Article Snippet: .. Shortly, pcDNA3.1 vector expressing the spike gene of SARS-CoV-2 lacking the C-terminal 21 amino acids, the full spike of SARS-CoV-1, or VSV-G (pMD2.G [Addgene #12259]), was co-transfected in HEK 293T cells with the murine leukemia retrovirus (MLV) expressing the NanoLuc luciferase gene and plasmid expressing the MLV Gag-Pol using Fugene HD transfection reagent (Promega). ..

    Article Title: DAZAP2 functions as a pan-coronavirus restriction factor by inhibiting viral entry and genomic replication.
    Article Snippet: .. Shortly, pcDNA3.1 vector expressing the spike gene of SARS-CoV-2 lacking the C-terminal 21 amino acids, the full spike of SARS-CoV-1, or VSV-G (pMD2.G [Addgene #12259]), was co-transfected in HEK 293T cells with the murine leukemia retrovirus (MLV) expressing the NanoLuc luciferase gene and plasmid expressing the MLV Gag-Pol using Fugene HD transfection reagent (Promega). ..

    Molecular Cloning:

    Article Title: Visualization of lysosomal membrane proteins by cryo electron tomography.
    Article Snippet: The sequences for human TRPML1 and human TRPML1-mNeonGreen1D4 were cloned into the mammalian expression vector pcDNA3.1 by Genscript. .. The pcDNA3.1 vector containing human TMEM192mCherry-1D4 was constructed by standard molecular cloning. pLJC5Tmem192-3xHA was a gift from David Sabatini (Addgene plasmid # 102930; (http://n2t.net/addgene:102930); RRID:Addgene_102930)21. ..

    Article Title: Visualization of lysosomal membrane proteins by cryo electron tomography
    Article Snippet: The sequences for human TRPML1 and human TRPML1-mNeonGreen-1D4 were cloned into the mammalian expression vector pcDNA3.1 by Genscript. .. The pcDNA3.1 vector containing human TMEM192-mCherry-1D4 was constructed by standard molecular cloning. pLJC5-Tmem192-3xHA was a gift from David Sabatini (Addgene plasmid # 102930; ( http://n2t.net/addgene:102930 ); RRID:Addgene_102930) . ..



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